Glucose is the preferred carbon source for Escherichia coli bacteria. Researchers isolated E. coli-M, a strain of E. coli with a single mutation that inactivates a key enzyme in one of the three glycolytic pathways E. coli bacteria use to break down glucose. In an initial experiment to study the effect of the mutation on glucose utilization and ATP production by the bacteria, the researchers add 2×104 E. coli-M bacteria to 400 mL of a growth medium that contains glucose. To measure the rate of bacterial growth, they monitor the optical density of the culture as a measure of the bacterial concentration over time.

Glucose is the preferred carbon source for Escherichia coli bacteria. Researchers isolated E. coli-M, a strain of E. coli with a single mutation that inactivates a key enzyme in one of the three glycolytic pathways E. coli bacteria use to break down glucose. In an initial experiment to study the effect of the mutation on glucose utilization and ATP production by the bacteria, the researchers add 2×104 E. coli-M bacteria to 400 mL of a growth medium that contains glucose. To measure the rate of bacterial growth, they monitor the optical density of the culture as a measure of the bacterial concentration over time.

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Monitoring the growth rate of E. coli- M bacteria is the useful indicator of the effect of glycolytic enzymes mutation on the bacteria because glucose Is the source of energy and ATP prod

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